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Hi Jean,
I'm guessing a wee bit here but I think as long as the cotton balls still smell strongly of peppermint, they're still viable. I got Mouse Magic at an Ace Hardware store. Not sure if Ace is all over the country or not but MM can also be ordered on line from a number of places...when googled, lots of options come up......more now than back when I got the stuff.
A friend has also used MM, in this case in her car engine because the mice were setting up shop in there, and she's had no problems since. It's probably not 100% effective but does seem to make a difference.
The MM packets smell of peppermint and spearmint and I would think that once that scent becomes minimal, it would be a good time to replace. How long that takes would likely vary depending upon the "weather" conditions the packet is in. Which reminds me, I'll have to check the "status" of my MM packets!
JJ