I imagine they'll know something is different, but I wouldn't think they'll get lonely since you have two of them. Maybe they'll like the break since the roosters are trying to mate with them.
My pekin ducks are 4 months old and have been free ranging with two roosters since they could start living outside. They follow each other around all day, the roosters keep gard while the ducks sleep. But they also fight and the roosters try to mate with my ducks. Im re-homing my two roosters in a few days and I'm wondering if my pekin ducks are gonna get lonely and not know what to do! Am i just overacting?