This is the most stressful time of incubating. Don't open the incubator for anything. And I think it would be better to try 3 times a day next time because the mother hen turns them up to 95 times a day, also, if you turn them an odd # of times a day then the embryo won't be on the same side of the egg during the night (which is the longest time it goes w/o turning in artificial incubation).