Thank you for the added details—it helps to get a clearer picture of the situation.
I’m so sorry for what you’re going through with your flock.
Going over your situation again, likely causes...Marek's Disease. The classic signs you mentioned—fluffing up, lethargy, and losing control of the neck—can occur with Marek's in some forms (neurological or visceral). However, Marek’s usually progresses over a longer period than just 48 hours.
Botulism is still possible. Rapid onset of symptoms, such as losing neck control (also called "limber neck") and not being able to move, could indicate botulism, which is caused by toxins from decaying organic matter (like spoiled feed, wet scratch, or a hidden carcass).
Check for wet or moldy feed, or areas where they might have ingested contaminated material.
Then back to cold stress or secondary Illness. The sudden drop in temperature could have made them susceptible to infections like mycoplasma gallisepticum or infectious coryza.
These often present with symptoms like puffed-up feathers, lethargy, and sometimes respiratory signs (though not always).
Cold stress combined with any underlying condition (like latent Marek’s) can cause sudden, severe illness.
We might also think about toxins or poisoning. If they were exposed to something toxic (e.g., pesticides, rat bait, or certain plants), it could explain the quick decline.
Try to rule out access to anything harmful in their environment.
Steps to take straight away:
1. Supportive Care for the Sick Hen. Continue Nutri-Drench and hydration with a syringe. Adding electrolytes to the water (or making your own with water, sugar, and itsy bit of salt) can help restore energy.
Try offering scrambled eggs or wet mash made with their layer feed for easy digestion.
If her neck control worsens, gently support her head when feeding or hydrating to prevent choking.
2. Quarantine and Observe the Others. Keep the sick bird isolated to prevent potential spread.
Watch the rest of the flock closely for early signs of illness, like puffed-up feathers, lethargy, or standing apart from the group.
3. Disinfect.
Thoroughly clean the coop and feeders/waterers. Remove any spoiled feed, wet scratch, or areas where toxins could accumulate.
4. Monitor for Botulism.
Look around for possible sources of botulism, like decayed organic matter or moldy food.
If you suspect botulism, you can try flushing their system with a molasses flush:
Mix 1 cup of molasses in 1 gallon of water and let them drink it (or syringe it gently if they can't drink on their own).
5. Consider Treatment for Respiratory Illness
While it’s hard to know for sure if this is a respiratory issue without other symptoms (like wheezing or discharge), a broad-spectrum antibiotic like Tylan 50 (injectable or water-soluble) could help if you suspect a bacterial infection.
Next Steps for the Flock
Consider Vaccination for Flocks
It’s excellent that your new flock is vaccinated. This will help reduce future losses from Marek's. Consider only adding vaccinated birds moving forward.
Sad part, but consider Necropsy if Possible.
If the sick hen doesn’t make it, (and I hope she does), a necropsy can provide valuable answers about whether Marek’s, botulism, or another illness is at play.
You could try contacting your local agricultural extension office or vet for guidance.
Given the quick decline and the combination of symptoms, botulism or stress-triggered Marek’s seem like the most likely culprits.
The best approach right now is supportive care for the sick bird, while focusing on prevention for the rest of the flock.
Sorry for how long that was. Wanted to be thorough. Hoping for the best for you and your girls!