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I would wait and see what happens. Later on today, candle the egg and see what it looks like, and/or do a float
test if there isn't a significant sign the egg is alive while candling. It may be fertile, but just didn't make it.
It happens.
Candling Pictures
Float test
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I wouldn't worry about it, she'll do her job. What happens, happens. Also, being you don't know if tomorrow
is day 21 or not, I've seen some hens 'lock down' on day 20. and some, not at all. If you lock her in the nest,
whatever you may use could possibly scare her, and she might accidently trample the eggs - trying to get out
of the nest (
Happened to me before, It was terrible) Is she a first time broody? If so, she may not be the
best mommy/broody. I noticed when my hens go broody for the first time, they get off the eggs alot, etc. Just
wait it out, and offer her food and water in the nest.