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No - you don't need to cull your flock if you believe your serama died from Marek's. From what I gather, Marek's disease is in most flocks anyway, but the vaccine helps prevent the symptoms. I'm sorry you lost your serama roo.
Yes, Mereks is latent in virtually all flocks. From Merck, "Every flock, except for those maintained under strict pathogen-free conditions, may be presumed to be infected." I've read about some of those pathgen-free flocks. HUGE biosecurity in a laboratory setting, year long quarantine to bring in birds, including eggs hatched in the quarantine, even when coming from another pathogen-free flock.
There are some forms more virulent than others, and if you see your birdsstart dropping like flies, you may need to do something, but not until then. Some birds are genetically more resistant or less resistant to Marek's (has to do with their blood type).
Anytime you have a question about chicken health, the Merck Veterinary Manual has an online edition that is quite good, and frequently updated: merckvetmanual.com. For Marek's, click on poultry, then on Neoplasms, then select the hyperlink you want.
Diagnosis of Merek's requires sighting of certain types of tumors in certain locations; I do not think this could be done without necropsy. There are some typical symptoms of Mareks, but they may also be symptoms of other ailments as well. For example, inability to walk or weak wings can be assorted with aspirgillosis, botulism, electrolyte imbalance, starvation, etc.
If you are truly convinced that the bird was ill, remove all bedding, and clean everything thoroughly and then apply a strong disinfectant (oxine, virkon-s, tek-trol, novalsan, etc.) according to instructions.
for your loss
