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Don,
Just from what I've experienced here with the carnation, it takes two sets for them to express it. Since all 4 of the older pullets have a carnation comb and they all came from a brother/sister mating, they got one set from each parent. Now with this younger pullet, she hatched out 3 weeks later than the others, so I'm thinking by then, the old roo's sperm was out of the mix, and this may be a Clyde baby. She does not, so far, have the carnation. So, she would only have the one gene from the mom's side. If her babies end up with a carnation when breeding her back to Clyed, that would tell me that he also carries one gene for it. See what I mean? I've only got one bird here with a sprig, and he's not been involved with any hatchings.
If the pullet has the mother with Carnation Comb, I would not keep her for breeding regardless if she produces a Carnation comb or not. You already know she is from a hen that has a carnation comb. From everything I have been told by Penne people usually the crosses with Marans you will get mostly the males with Carnation and a very few female. The reason you got so many is possibly because you had a double dose going on.
Don,
I don't believe she is from the hen
with the carnation, but from one of the sisters to her. I can see in the chicks that have the carnations, which ones (2) probably came from the hen who does have it, their's are huge, like a double whammy! The other two original hens don't express the carnation, so I figure only carry the one gene for it. This pullet will be my "comb tester"!