New Way to watch an Embryo Grow Try#4 (Last Video)

[pax12]

Thanks you so much I cant wait to start working on the next one in a few days.

 

[Roccomanchickens]

Good job with your success so far (it was successful in that you learned something) Keep thinking outside the status quo and good luck!

 

[JayneMae]

i am also VERY interested in this! very intriguing!

 

[pax12]

im getting 4- 8 eggs ducks and chicken tomorrow, but I have to wait for the bags to arrive.
Im going to try every experiment over again.

 

[pax12]

Fertile Egg In Bag Experiment Try #4
Im trying to find a new way to hatch and grow an embryo.

Old videos of my experiments http://www.youtube.com/playlist?list=PL999FF131D6698E1E

I am going to try Kordon breathing bag... it should allow gas exchange but maintain moisture level. They are used for shipping fish and they have tested them with some fish for several weeks. The bag is a membrane that allows oxygen in, CO2 out, and keeps the liquid inside.
and ground up eggshells (calcium) on the yoke and white.

Videos-

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[Redcatcher]

Interesting. It's been done before in an experiment to see if birds can be cloned in the near future. If you want, I can dig around and try to find the article.

 

[Redcatcher]

I had no idea it was this simple. It's a high school or college level project with easily obtained materials.

http://www.ableweb.org/volumes/vol-5/8-fisher.pdf


Introduction
This method, referred to by students as “chicks-in-plastic,” “chick-in-a-cup,” or “the omelette
lab,” allows continuous observation of living chick embryos from day 3 to day 18 of incubation.
Differentiation of organ systems and extraembryonic structures as well as pre-hatching behavior can
be studied by students from grade school through college. For very young students, a
demonstration should be set up by the instructor for the class to observe. In high school or college
introductory biology or embryology courses, students can study developmental processes and also
practice in vitro culture techniques. For advanced developmental biology courses, project-oriented
studies of growth (Dunn and Boone, 1976), teratology (Pearson, 1983), calcium metabolism (Burke
et al., 1979; Dunn et al, 1981; Narbaitz, 1979; Narbaitz and Jande, 1978; Tuan, 1980), angiogenesis
(Castellot et al. 1986; Dobson et al., 1990; Sherer and Dostal, 1982), or osteogenesis can be
devised.
Early attempts to grow chick embryos outside their shells (Boone, 1963; Quisenberry and
Dillon, 1962) met with limited success. Bruce Dunn (1974), a high school student working in an
improvised basement laboratory, devised a method for growing chick embryos in plastic slings.
Working with Boone, Ramsey and Dunn improved shell-less embryo culture methods (Dunn and
Boone, 1976; Ramsey and Boone, 1972). Castellot et al. (1982) simplified the method further by
substituting disposable hot cups (“chick-in-a-cup”) for plastic tripods.
Since Dunn's publication of his method in 1974, it has fascinated grade school children, won
high school Science Fair Awards, and served as the experimental model for published scientific
research. The simplicity, adaptability, and elegance of this method make it a pedagogical delight.
In a world of rapidly changing technology and expensive equipment, the success of this method
demonstrates that ingenuity and brains are more productive than button-pushing for good research
and teaching.
Materials

 

[pax12]

Candling Chicken and Duck Day 7

 

[Stacykins]

Quote:
A kordon breather bag (I've used them for shipping fish so I know what they are) won't work because it isn't sterile. I posted before, the inside of an egg is a sterile environment. The shell protects the embryo from pathogens, since the proteins and fats that make up the egg a bacterial buffet if they make it in.

I can see powered calcium causing harm because, surprise, not sterile!

 

[pax12]

I know but im going to still try and see what happens.