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I was doing great all winter but come summer I keep getting a weird mold on the seeds. The mold is bluish with a skinny stalk and a round head as viewed under the microscope. Not all trays all the time have mold so I'm suspecting that I need to sterilize all the trays. I tried cutting back the volume of seeds so they could dry better but that's not working. I tried adding bleach to the soak water but that did nothing.
I'm all ears...
JT
think the "bad" bag of barley I got was maybe older stock and already was full of mold type spores that came to life when I started watering those seeds.
Randall P Niedz
United States Department of Agriculture
A couple of things come to mind -
1) Vary bleach concentration and time and see if that helps - this captures the inverse relationship between bleach concentration and time (i.e., high concentration/shorter time vs low concentration/longer time). I have found this strategy useful when the tissue is damaged by the bleach concentration.
2) Related to #1 is to use acidified bleach (essentially dropping the pH using an acid). Acidified bleach is extremely potent. For example, I add 10 mls bleach (5.25% sodium hypochlorite) + 10 mls vinegar (5% acetic acid) to 19 liters of water to sterilize bottles for home brewed beer- I soak them for 15 mins. This is only 0.007 M chlorine. In the lab I will start with 10% bleach and neutralize with an NaOH solution, then vary the incubation time as described in #1.
3) A short acid treatment (e.g., 1N HCl or H2SO4) followed by a dip in a dilute base (e.g., 0.1N NaOH) to neutralize the acid. I generally use this preceding a bleach treatment.
4) Heat treatment. Seeds are soaked for 4-24 hours, then placed into sterile cloth bags, and the bags immersed into hot water (45-60C) for 5-10 mins. Because pecans are large, longer immersion may be necessary. I haven't tried this but found it interesting - Watts, J. E.; De Villiers, O. T.; Watts, L., 1993: Sterilization of wheat seeds for tissue culture purposes. South African Journal Of Botany. 59(6): 641-642.
Cite
I never thought about the mold being on the seeds from the get go. Some research shows a lot of universities use a 30 minute soak in 5% Sodium Hypochlorite followed by several rinses in sterile water.
For me the only way my CQ's get greens is sprouts so it's worth the effort to grow.
I'd like to see your system.
JT