Chiming in here, sounds like you've gotten great help/advice already. Yes to be on the safe side, do a re-treatment and keep an eye on your birds for any signs of new lice or egg clusters....they like to hang out in warm areas of the body like under the wings and around the vent FYI...I'm surprised you weren't able to find permethrin...I used to work at a feed store and it is one of the most common pesticides out there....they use it in human lice shampoos as well I believe...it's basically a synthetic form of a naturally occurring pesticide found in certain strains of Chrysanthemum called "pyrethrin" (if you care to know, lol) and Bonide (a common pesticide manufacturer) sells this chemical in sprays, concentrates and powders called "Eight". I have treated my girls for pest infestations before, and have never had to turn them upside down...I have always just pinned them in a squatting position, and with a gloved hand fluffed as much of the dust as I can under their feathers taking care to not get it in their faces. I would also remove any bedding, sprinkle dust, or use a spray on the cracks and crevices of your coop and roosts, and then place new litter on top. Some nice preventative measures to keep in mind are to keep bedding and nests as clean and dry as you can and your run mud and muck free (sand is a stellar material for dryness and drainage) It sounds like you make your own dust baths? Sand, Wood ashes (from untreated and unpainted woods) and Diatomaceous Earth are great materials to include. DE is great at killing all kinds of insects, but becomes inert when wet, so permethrin is better for infestations and DE is great as routine inclusive in dust baths. I sprinkle a little in my girls' favorite holes from time to time. More info than you wanted? Perfect. Cheers!