Here are the results...She was diagnosed with a type of "Lymphoproliferative disorder; with aggregates of homogenous lymphoid cells that have mild anisocytosis and
anisokaryosis with severe multifocal lymphoproliferative meningitis and perivascular encephalitis of the brain and spinal cord. The cause for the severe neurologic progression and cellular proliferation is attributed to a neoplasia-producing virus" similar to but not typical of LL or MDV:
ID ID Type Taxonomy Gender Age
S p e c i m e n D e t a i l s
CAHFS Internal ID Chicken Female 21.00 Weeks
Adult hen; history of recurrent, progressive neurologic condition:
Microscopic Findings:
1. Severe lymphoid cellular infiltration of brain and spinal cord meninges and blood vessels, liver, spleen, small
intestine, lung, thyroid gland, skeletal and smooth muscle, pericardium.
Gross Findings:
1. Multiple small firm, pale nodules in liver.
2. Solitary transmural thickened nodule in small intestine.
Test Results:
1. Bacteriology/parasitology:
a. Large number coccidia oocyts found in colon/cecum contents.
b. No pathogenic bacteria isolated from internal organs, intestine.
2. Virology:
a. Negative for Avian Influenza virus by qRT-PCR.
3. Toxicology:
a. Slightly elevated zinc level in liver.
b. Adequate, non-toxic additional minerals levels.
L a b o r a t o r y F i n d i n g s / D i a g n o s i s
Final Report (02/13/10): Severe lymphoproliferative cellular infiltrates were found in the meninges and vessels within the
brain, spinal cord, and multiple internal organs. The sciatic nerves and brachial plexus nerves were normal. The cause
for the severe neurologic progression and cellular proliferation is attributed to a neoplasia-producing virus.
C a s e S u m m a r y
Report 4(q)-CAHFS Standard Report- 2/10/2010 Page 1 of 4
CAHFS Final Version February 14, 2010
Additional tests - Negative for Avian Influenza virus by qRT-PCR. Liver zinc concentration slightly elevated but
non-toxic. No pathogenic bacteria isolated from internal organs or digestive tract. Testing is complete for this case.
G r o s s O b s e r v a t i o n s
Necropsy of an 880 gram black and white spotted adult hen began at 11:30 am, February 5, 2010. The specimen is
submitted live, and humanely euthanized. The specimen exhibits neurologic disorder clinical behaviour, whereby the
head is poorly controlled by the neck musculature, the head drops and twists, and the neck twists irregularly. The
specimen is mildly obtunded.
The specimen is markedly thin, with prominent keel bone exposure. Feather coat is clean and full. Internal fat reserves
are depleted. The liver contains multifocal pale tan firm plaques to nodules, up to 5mm in diameter, that are scattered
throughout the liver parenchyma and surface capsule. There are > 20 nodules. There is a focal solitary nodule of the
duodenum within the duodenal loop, approximately 15mm in diameter, where the duodenal walls are markedly
thickened by firm homogenous tissue. There is scant feed within the crop, proventriculus and gizzard. The small
intestine contains scant dark green mucoid digesta. The ceca and colon contains dark green pasty to semifluid digesta .
The ovary is small and inactive. The spleen is of expected size. The brain and cranium are unremarkable. No additional
lesions are noted in remaining organ systems.
B a c t e r i o l o g y
Animal/Source Specimen Specimen Type Date Resulted
Salmonella PCR and Culture
Results
1 Intestinal Tissue Pool 10-Feb-2010 No salmonella detected
Animal/Source Specimen Specimen Type Date Resulted
BACTERIAL AEROBIC CULTURE
Results
1 Liver Tissue 09-Feb-2010 No growth after 48 hours
1 Lung Tissue 09-Feb-2010 Mixed flora Rare
B i o t e c h n o l o g y
Animal/Source Specimen Specimen Type Date Resulted
Avian Influenza matrix gene qRT-PCR
Results
Chicken Cloacal Swab - VTM 10-Feb-2010 Negative
1 Pharyngeal Swab -
VTM
Chicken 10-Feb-2010 Negative
Report 4(q)-CAHFS Standard Report- 2/10/2010 Page 2 of 4
CAHFS Final Version 1 February 14, 2010
H i s t o l o g y
Tissues sampled: brain, liver, kidney, lungs, spleen, ovary, sciatic nerves, brachial plexus, trachea, larynx, heart,
proventriculus, gizzard, small intestine, pancreas, cecum, colon.
Findings:
1. Lymphoproliferative disorder; with aggregates of homogenous lymphoid cells that have mild anisocytosis and
anisokaryosis, and 1-2 mitotic figures per high-power field:
a. Brain: severe, multifocal lymphoproliferative meningitis and perivascular encephalitis.
b. Spinal cord: severe, multifocal lymphoproliferative meningitis.
c. Additional organs - liver, spleen, thyroid gland, kidney, lungs, skeletal muscle, smooth muscle, small intestine.
No additional lesions are noted in remaining examined tissues.
P a r a s i t o l o g y
Animal/Source Specimen Specimen Type Date Resulted
FECAL EXAM - FLOTATION
1 Feces 05-Feb-2010 Coccidia oocysts Lge#
T o x i c o l o g y
The submitted liver had the listed metals in acceptable, non-toxic concentrations for chickens. Please note that the zinc
concentration is slightly elevated, but non-toxic.
Reporting limit (Rep. Limit): The lowest routinely quantified concentration of an analyte in a sample. The analyte may be
detected, but not quantified, at concentrations below the reporting limit.