Research has shown the male embryo is more vulnerable to cold temps than the female. The males die in the shell.
They were stored in a refrigeratorI'm not sure what you mean by "cold storage method." Generally, the eggs I'm going to incubate are kept in the basement until I have enough. It's about 65°F down there, instead of 70°F upstairs. A couple of times, I didn't have enough, so I grabbed some out of the fridge. They were in the fridge for anywhere from a few days to three weeks. My hatch rate was definitely down a bit, but most hatched. I didn't keep them, so not sure of the sexes of any.
Brinsea even recommends that if we have a power outage expected to be two or more hours and we're in the first 0-14 days of incubation, to put the eggs in the refrigerator. I would not do this though and would just cover the incubators. We also have a generator we'd get running to power up the incubators.
https://www.brinsea.com/t-poweroff.aspx#:~:text=If you experience a power,More Information & Species Differences
