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What kind of Temperatures are you having where you live and are the any wild animals or dogs running around that could be upsetting you ladys .
Your feed is most assume as not everyone feeds there poultry so well ..
I have a couple of ladys that were returned to me after the original purchaser had an attack that left two of the ladys missing and the other two haven't produced and eggs in the last three months but they are not as on edge and are willing to let me pick them up but trauma effects each animal differently so now I need to ask can you pick up or touch these ladys with out them freaking out .