In OVO vaccination

duluthralphie

Dux eradication specialist
8 Years
Jul 11, 2014
40,472
114,267
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Orrock township, Minnesota
I have been reading a lot of info on in ovo vaccination for Mareks.

I do not have a $50K machine to use.

I am wondering if anyone has tried this on their own by hand. I am thinking poking a hole in the shell (I think above the air sac and dropping the vaccine in might work. At day 18-19 I doubt the hole would kill the chick if the humidity is high in the hatcher.

Any thoughts? I am not going to do this with this hatch, but I might try a hatch just to see what the results would be...
 
I would drill a small hole in the middle above the air sac and drop the vaccine onto the membrane.

I am thinking I could seal the drill hole with a piece of tape. But am not sure that would be needed as the humidity would be +-5 of 70.

This is an article I found it lists the areas the vaccine can go, the air sac makes the most sense for an untried person like me with no fancy machine other than a dremil.

https://www.egginject.com/In-ovo-vaccination
 
I have decided this is even more possible than I originally thought.

Those silly call dux people make holes in the eggs all the time which might be why they have such low hatch rates. Of course, that could be the humanity of them trying to save the world from DUX, but I digress.

If they can punch a hole in the egg I should be able to also and get the vaccine in.

I will try to get one of the deranged DUX owners to come here and tell us how they poke the hole in the egg.
 
I would drill a small hole in the middle above the air sac and drop the vaccine onto the membrane.

I am thinking I could seal the drill hole with a piece of tape. But am not sure that would be needed as the humidity would be +-5 of 70.

This is an article I found it lists the areas the vaccine can go, the air sac makes the most sense for an untried person like me with no fancy machine other than a dremil.

https://www.egginject.com/In-ovo-vaccination
Since it recommends doing the vaccination at 18 days of incubation, there is no need to seal the hole that is made for the injection.

I have made the hole into the air cell by gently twisting a tack or small drill bit against the shell by hand. The hole has helped me in hatching shipped Cream Legbar eggs.
 
According to the bar graph in that study, air cell injection does not result in an appropriate uptake of the vaccine to be effective. The study indicates that the vaccine must be delivered directly into the amnion or into the chick it'self. That being the case, if I were vaccinating, I'd rather do it on a newly hatched chick than be drilling an egg, and hoping that I was able to hit the correct spot with the needle without rupturing a major blood vessel or causing injury to the chick.
 
Since it recommends doing the vaccination at 18 days of incubation, there is no need to seal the hole that is made for the injection.

I have made the hole into the air cell by gently twisting a tack or small drill bit against the shell by hand. The hole has helped me in hatching shipped Cream Legbar eggs.
Cool!


Thanks

How does the hole help eggs hatch?
 
According to the bar graph in that study, air cell injection does not result in an appropriate uptake of the vaccine to be effective. The study indicates that the vaccine must be delivered directly into the amnion or into the chick it'self. That being the case, if I were vaccinating, I'd rather do it on a newly hatched chick than be drilling an egg, and hoping that I was able to hit the correct spot with the needle without rupturing a major blood vessel or causing injury to the chick.

You're right.

I am still thinking I want to try it.

Trying to get the vaccine just under the skin in a day old chick is not exactly fun. They move and the skin is so thin the needle will sometimes come out the other side of the skin.

I am not sure where to inject otherwise. If a machine can do it a person should be able to.
 
i found a little bit, slight cone shape helps keep from drilling too far. I just twirl it in with my fingers.
I do crafts with them too. Embroidery on eggs. :D
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