PeeWee is sick! UPDATE..FINAL Necropsy Results Page 6

Discussion in 'Emergencies / Diseases / Injuries and Cures' started by Leslieb118, Jun 3, 2012.

  1. Leslieb118

    Leslieb118 Songster

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    I got a lot of great advice while trying to treat my little guy. It's the least I can to to follow up, because hopefully it will help the next person. As soon as the lab work comes back, I'll post the results here, and hopefully we'll have some answers! Thanks!
     
  2. Leslieb118

    Leslieb118 Songster

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    University of Kentucky Veterinary Diagnostic Laboratory
    Dr. Lynne Cassone


    ~';[nlil ReQort.. _
    Case Coordinator: Veterinary Diagnostic
    Laboratory
    Dr. Lynne MCassone

    Submitted Date: 6/12/20123:09 PM
    Collection Date: 6/11/2012
    Accession No: K12009319
    LESLIE BURTON
    Phone: (502) la_.-
    Fax:
    Email:
    LOUISVILLE KY..-
    Associated Parties
    Owner, Other: Leslie Burton
    Reference Data
    Number Of Dead: 1
    Number In Group: 18
    Number On Farm: 18
    Animal Information
    Pee Wee; Phasianidae - Chicken; Male; 10 Months
    History
    General
    Left eye drained cloudy thin fluid. Comb, wattles & face turned pale. After a few days, was lethargic, unsteady on feet,
    losing tremendous amounts of weight, des ite eating and drinking normally. Droppings normal until 6/10/12.
    Nutrition
    Purina flock raiser, free range, ACV in water.
    Responses
    continued to deteriorate.
    Treatments
    dusted w/sevin, wormed wlWazine & Valbazen - Tylan 50 injectable given orally 1/4cc per day.
    Diagnosis
    Lymphoproliferative disease (presumed);
    Acute bacterial bronchopneumonia;
    Acute necrotizing proventriculitis.
    Diagnosis Comment
    Unfortunately, the cellular infiltrate in many of the tissues could not be characterized due to a combination of autolysis
    and freeze artifact. Given the widespread distribution, the most likely pathogenic process is a virally-induced
    Iymphoproliferative disease, such as Mare~'s Disease or Lymphoid Leukosis.
    Bacterial infection of the eyes, upper respibtory tract, and lungs is due to infection by opportunistic bacteria (£ coli,
    Staphylococcus sp.). The lesions of the proventriculus are also presumed to be secondary to Iymphoproliferative
    disease, based upon the presence of large numbers of cells throughout the wall of the proventriculus, apart from the
    ulcerated surface.
    Pathologic Anatomic Findings

    GROSS EXAMINATION:
    A young adult, black and white cockerel in poor body condition is submitted .. Autolysis is moderate, and the carcass had

    Page 1 of 4 - Final (6/27/2012) - Accession No: K12009319


    University of Kentucky Veterinary Diagnostic Laboratory
    Dr. Lynne Cassone

    been frozen.
    The choanae are covered with a mucoid exudate. The lungs are congested and discolored pale tan. The mucosal
    surface of the proventriculus is coated by a fibrinous exudate.
    HISTOLOGIC EXAMINATION:
    Autolysis and freeze artifact limit histologic interpretation.
    Proventriculus: Severe, subacute to chronic, transmural, mixed mononuclear proventriculitis, with ulceration, superficial
    necrosis and heavy superficial bacterial colonization.
    Ventriculus: Moderate, subacute, mixed inflammatory serositis.
    Liver: Moderate, multifocal, periportal nucleated cell infiltrate (cell type could not be discerned).
    Lung: Marked interstitial nucleated cell infiltrate (cell type could not be discerned).
    Kidney: Marked interstitial nucleated cell infiltrate (cell type could not be discerned).
    Esophagus: Moderate hyperkeratosis.
    Heart, Spleen, Pancreas, Colon: No significant lesions observed.

    Lab Fil1dings
    Bacteriology
    Specimen
    Test Name
    Organism
    Quantitation
    Pee Wee - Avian - Phasianidae - Chicken - Male - 10 Months
    Lung - Tissue, fresh - 3 Culture-Aerobic (Culture - AERCUL) - 6/18/2012 10:46 AM E. coli Few
    Staphylococcus (coagulase negative) Rare
    Nonpathogenic bacteria
    Choana - Swab - 4 Culture-Aerobic (Culture - AERCUl) - 6/18/2012 10:46 AM E. coli Numerous
    Nonpathogenic bacteria
    Eye - Swab - 5 Culture-Aerobic (Culture - AERCUL) - 6/18/2012 10:46 AM E. coli Numerous
    Staphylococcus (coagulase negative) Numerous
    Nonpathogenic bacteria

    Specimen Test Name
    Organism
    Drug
    MIC
    Interpretation
    Pee Wee - Avian - Phasianidae - Chicken - Male - 10 Months
    Eye- MIC Panel (Sensitivity - MIC) - Escherichia coli Amoxicillin 4 Susceptible
    Bacterium - 6/18/201210:46AM
    9319-1
    Escherichia coli Ceftiofur <=0.2500 No Interpretation
    Escherichia coli Clindamycin >4.0000 Resistant
    Escherichia coli Enrofloxacin <=0.1200 Susceptible
    Escherichia coli Erythromycin >4.0000 Resistant
    Escherichia coli Florfen icol 2 Susceptible
    Escherichia coli Gentamicin 1 Susceptible
    Escherichia coli Negative Growth Control 1 Valid Growth Control
    Escherichia coli Neomycin 4 Susceptible
    Escherichia coli Novobiocin >4.0000 Resistant
    Escherichia coli Oxytetracycline 2 Susceptible
    Escherichia coli Penicillin >8.0000 Resistant
    Escherichia coli Sulphadimethoxime 128 Susceptible
    Escherichia coli Spectinomycin 16 Intermediate
    Escherichia coli Streptomycin <=8.0000 No Interpretation
    Escherichia coli Sulphathiazole <=32.0000 Intermediate
    Escherichia coli Tetracycline 2 Susceptible
    Escherichia coli Trimethopriml <=0.5000 Susceptible
    Sulphamethoxazole

    Page 2 of 4 - Final (6/27/2012)
    - ACfesslon No. K12009319

    Specimen Test Name
    UKY
    University of Kentucky Veterinary Diagnostic Laboratory
    Dr. Lynne Cassone

    Organism Drug
    MIC
    Interpretation
    UK.
    Escherichia coli Tylosin (Tartratel Base) >20.0000 No Interpretation
    Eye- MIC Panel (Sensitivity - MIC) - Staphylococcus species Amoxicillin <=0.2500 No Interpretation
    Bacterium - 6/18/201210:46 AM
    9319-2
    Staphylococcus species Ceftiofur 1 No Interpretation
    Staphylococcus species Clindamycin >4.0000 Resistant
    Staphylococcus species Enrofloxacin <=0.1200 Susceptible
    Staphylococcus species Erythromycin >4.0000 Resistant
    Staphylococcus species Florfenicol 4 Intermediate
    Staphylococcus species Gentamicin <=0.5000 Susceptible
    Staphylococcus species Negative Growth Control 1 Valid Growth Control
    Staphylococcus species Neomycin <=2.0000 Susceptible
    Staphylococcus species Novobiocin <=0.5000 Susceptible
    Staphylococcus species Oxytetracycline >8.0000 Resistant
    Staphylococcus species Penicillin <=0.0600 No Interpretation
    Staphylococcus species Sulphadimethoxime <=32.0000 Susceptible
    Staphylococcus species Spectinomycin 64 Resistant
    Staphylococcus species Streptomycin <=8.0000 No Interpretation
    Staphylococcus species Sulphathiazole <=32.0000 Intermediate
    Staphylococcus species Tetracycline >8.0000 Resistant
    Staphylococcus species Trimethopriml <=0.5000 Susceptible
    Sulphamethoxazole
    Staphylococcus species Tylosin (Tartratel Base) >20.0000 No Interpretation
    Comment: The numbers represent the Minimum Inhibitory Concentration (MIC) as ~g/mL, which is the lowest
    concentration of antimicrobial agent required to inhibit the growth of a bacterial isolate.
    The antimicrobial susceptibilities reported are based upon the most recent Clinical and Laboratory Standards
    Institute (CLSI) Guidelines. For antimicrobial drugs not approved for use in poultry, the laboratory client or
    veterinarian assumes all responsibility for efficacy, safety, and residue avoidance with extra-label use of
    those reagents.

    Clinical Pathology
    Specimen Test Name
    Parasites

    No ova or parasites observed

    Molecular Biology
    Specimen Test Name Result
    Pee Wee - Avian - Phasianidae - Chicken - Male - 10 Months

    Trachea - Swab - 7 IL T (Real Time PCR - ILTPCR) - 6/20/2012 11 :58 AM Not Detected
    Client Report History
    Page 3 of 4 - Final (6/27/2012)
    - Accession No: K12009319
     
    Last edited: Jun 30, 2012
  3. Leslieb118

    Leslieb118 Songster

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    OK..whew! So, does anybody have any idea what all of this means? The text in Red I have questions about, and the text in bold black I just wanted to emphasize. He had no worms. Unfortunately, I had put him in a cooler with dry ice since UPS didn't want a leaking package, and he was partially frozen by the next day when he arrived at the lab.
     
    Last edited: Jun 29, 2012
  4. The Lazy L

    The Lazy L Songster

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    the most likely pathogenic process is a virally-induced Iymphoproliferative disease, such as Mare~'s Disease or Lymphoid Leukosis.
     
  5. CMV

    CMV Flock Mistress

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    I am so sorry. You have Marek's in your flock. Any birds you acquire from this point forward will need to be vaccinated.

    At least you know the reason he passed. And you know that there was really nothing you could have done to save him.
     
  6. Leslieb118

    Leslieb118 Songster

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    I'd never had suspected that at all when I was trying to treat him. While waiting for the report, I scoured this one page I came across, looking at symptoms, pictures, all those things and still couldn't come up with anything. Now that I've gotten the report back, it's proving somewhat useful in looking up the exam findings. It's a really neat site.

    http://partnersah.vet.cornell.edu/avian-atlas/

    I'm curious to know if TSC sells vaccinated or unvaccinated chicks. I bought 8 chicks from them in March. I also hatched out others. They weren't vaccinated. I've got 4 chickens left from my original flock with a total of 17 now. Guess I've got a lot of reading to do.
     
  7. Adenium

    Adenium Songster

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    As far as I know, none of the feed stores get vaccinated chicks. Not everyone wants it, and while it's inexpensive overall it would probably eat into their profit margin.
     
  8. seminolewind

    seminolewind Flock Mistress

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    I agree with CMV. I am going thru exactly the same thing right now. And the birds that are dying were vaccinated 2X for Marek's!

    The roo that died yesterday had both eyes swollen shut just like yours. And died. Three died a month ago from coccidiosis, probably one of those opportunistic bacteria. Because of the swollen eyes, I was not looking for Marek's, then learned from a friend that Marek's leaves a bird open for opportunistic bacteria.

    Your's shows a few opportunistic bacteria. I'm glad you had the necropsy and posted the results. Even those who don't reply to these threads, there are thousands who don't, but learn about Marek's because they are reading these threads.
     
  9. Adenium

    Adenium Songster

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    I wonder if there was a different Marek's strain, Seminole. And is there any way to test for how well the vaccine has taken, like we can check titers for ourselves?

    This makes me worried, as I paid my nice $ for supercooled transport of my vaccine from Alabama and went through the trauma of giving the shots to my little flock. It kills me to think it may not be conferring much protection - though I knew it isn't 100%.
     
  10. Leslieb118

    Leslieb118 Songster

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    I did look up Lymphoid Leukosis, as it was mentioned in the report as a possibility other than Mareks, and it seems the symptoms fit that better..with the pale comb and wattles, and the weight loss. Either way, it seems they're in the same family of diseases.

    Seminolewind, I'm sorry you're going through the same issues. [​IMG]
     

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