Was this fresh or saltwater?Hello, I want to share an experiment I did with a small brute trash bin filled with bio balls and pure ammonia from Walmart in a 300 gallon plastic tank. I filled the tank with water and put in about a cup of ammonia and let the tank cycle through the nitrification process. I kept adding ammonia as soon as it was cleared, eventually the water ran out of KH minerals and the nitrification stopped clearing the ammonia. Then I put baking soda in the water and the nitrification started again. I concluded that the best test to get is the KH test. Because when the KH goes to zero your fish will get sick.