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I could debate this but I'm tired. The general rule is once they are exposed to an environment it's too late. Amount of time doesn't matter. You must assume exposure.Oh I see what you mean.
Technically from 3 weeks they were free to come and go as they chose - but I don't know that they spent that much time on the coop floor until about 4-5 weeks - but then they definitely did spend time on the old litter.
I think the theorists would say that the ideal thing would be to give them exposure in small doses rather than a full onslaught - so they can develop their immunities. Practically that might look like running around for a few hours during the day but still having their own environment for the rest of the time. I have never heard of anyone doing that in practice but it probably is the ideal 'integration' into their new microbiological environment!
Now in reality there is something called infective dose. You can be exposed but not pick up a large enough dose for an infection to develop.
What that means practically is that when I had a respiratory infection going through my tribe I could try quarantine hoping that not all had received an infective dose even though they had all been exposed.
Microbiology tax