I follow their instructions for that incubator, you should be fine. Yes, fill both sides for lockdown. I don't know about the weight loss, I don't weigh my eggs.I think its to late to do anything to mine. They are due to hatch on the 19th. What percentage of weight loss should they have at lock down? I candled last night and all are wiggling. I'm using a Brinsea Mini Advanced and through hatch ave kept water in one of the reservoirs per the instructions. It said to fill both sides. Its my first time using this incubator.